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Part:BBa_K771007:Design

Designed by: Guo Huaqing   Group: iGEM12_SJTU-BioX-Shanghai   (2012-09-25)


Membrane Anchor 2 without MS2:ssDsbA-LGT-SH3 Domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 883
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 504
    Illegal SapI.rc site found at 1150


Design Notes

For both Membrane Accelerator and Membrane Switch


Source

Lgt and ssDsbA are from E.coli genome; SH3 domain is obtained from Prof. John Dueber

References